Fig. 5
From: Universal CARs, universal T cells, and universal CAR T cells
Production of universal CD52−/TCR− T cells using TALEN (transcription activator-like effector nuclease). a The T cells were obtained from healthy adult donors. TALEN mRNAs targeting specific sites of CD52 and the constant region of the T cell receptor α chain (TRAC) were electro-transferred into the T cells at the same time leading to permanent loss of gene expression. These CD52−/TCR− T cells can be used for the preparation of “off-the-shelf” CAR T cells. b Diagram of TALEN-targeted sites. The blue band represents the chromosome. Scissors represent TALENs. The red blocks represent the targeted sites of TALENs. (adapted from Poirot, L, et al., Cancer Res. 2015; 75(18); 3853–3864)