Fig. 6
From: Universal CARs, universal T cells, and universal CAR T cells
Production of universal TCR−/HLA− T cells using the CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeat /CRISPR- associated protein 9). The T cells were obtained from healthy adult donors. Cas9 mRNA and gRNAs targeting the constant region of the T cell receptor αor βchain (TRAC or TRBC) and B2M were transferred to T cells by electroporation. The Cas9 endonuclease is capable of cleaving the TRAC, TRBC, and B2M under the guidance of corresponding gRNAs and inducing indels at pre-validated sites. These universal TCR−/HLA− T cells with reduced alloreactivity can be used for the preparation of “off-the-shelf” CAR T cells. (adapted from Ren, J, et al. Clin Cancer Res. 2016; 23(9); 2255–2266)