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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Universal CARs, universal T cells, and universal CAR T cells

Fig. 6

Production of universal TCR/HLA T cells using the CRISPR/Cas9 system (clustered regularly interspaced short palindromic repeat /CRISPR- associated protein 9). The T cells were obtained from healthy adult donors. Cas9 mRNA and gRNAs targeting the constant region of the T cell receptor αor βchain (TRAC or TRBC) and B2M were transferred to T cells by electroporation. The Cas9 endonuclease is capable of cleaving the TRAC, TRBC, and B2M under the guidance of corresponding gRNAs and inducing indels at pre-validated sites. These universal TCR/HLA T cells with reduced alloreactivity can be used for the preparation of “off-the-shelf” CAR T cells. (adapted from Ren, J, et al. Clin Cancer Res. 2016; 23(9); 2255–2266)

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