Fig. 8

MSCs-exo modulated the gene expression related to Th17 or Treg cells in vitro. a–d PBMCs were isolated from patients with active cGVHD and cultured udder a stimulative condition. Real-time PCR was utilized to analyze the mRNA expression of transcription factors. As expected, the MSCs-exo treatment suppressed the expression of RORrt (a) and STAT3 (b), whereas it promoted the expression of Foxp3 (c). No significant difference in T-bet (d) gene expression was observed. e, f ELISA was performed to detect the protein expression of IL-17 and IL-10. There was a significant reduction of IL-17 (a) and an elevation of IL-10 (f) in the MSCs-exo group compared to the PBS or Fib-exo groups. Data are expressed as the mean ± SEM. *P < .05, **P < .01, and ***P < .001; ns not significant