Fig. 2From: Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesisa Western blotting analysis of pEGFR and EGFR in whole lysates of RAW 264.7 cells incubated, for 6 days, with MM1.S derived exosomes (50 μg/ml) treated or not with anti-AREG mAb (50 μg/ml), with rhAREG (50 μg/ml) and rhRANKL (25 μg/ml). The histogram on the right represents the ratio pEGFR/EGFR, based on densitometric analysis normalized versus GAPDH, used as loading control. b Evaluation by quantitative real-time PCR of mRNA expression of SNAIL in RAW 264.7 incubated, for 3 and 6 days, with MM1.S-derived exosomes (50 μg/ml) treated or not with anti-AREG mAb (50 μg/ml), rhRANKL (25 μg/ml), and rhAREG (50 μg/ml). Human PB CD14+ cells incubated for 6 days in osteoclastogenic medium (rhRANKL 25 ng/ml and MCSF 25 ng/ml), with MM1.S derived exosomes (50 μg/ml) treated or not with anti-AREG mAb (50 μg/ml). *Exo vs untreated (**p ≤ 0.01; ***p ≤ 0.001); #Exo+AREG mAb vs Exo (#p ≤ 0.05)Back to article page