Fig. 6From: Multiple myeloma-derived exosomes are enriched of amphiregulin (AREG) and activate the epidermal growth factor pathway in the bone microenvironment leading to osteoclastogenesisa Analysis at confocal microscopy of hTERT-MSCs treated for 4 h with MM1.S exosomes pretreated or not with mAb AREG, compared with untreated hTERT-MSCs (Ctrl). hTERT-MSCs were stained with phalloidin Alexa Fluor (green), nuclear counterstaining was performed using Hoescht (blue), and exosomes were labeled with PKH26 (red); histogram shows fluorescence intensity expressed as ratio between a.u. and number of hTERT-MSCs treated with MM1.S exosomes and MM1.S exosomes pretreated with mAb AREG. b Levels of EGFR and phospho-EGFR were determined by FACS analysis in hTERT-MSCs after 48 h treatment with MM1.S exosomes pretreated or not with mAb AREGBack to article page