Fig. 3

SNHG6 affects CRC cell growth in vitro. a HCT-116 and HCT-8 cells transfected with the SNHG6 siRNAs were subjected to the CCK-8 assay. b HCT-116 and HCT-8 cells transfected with SNHG6 siRNAs were seeded into 6-well plates. The number of colonies was counted on the 14th day after seeding. c GSEA results were plotted to visualize the correlation between the expression of SNHG6 and genes related to cell proliferation (BENPORATH_PROLIFERAYION, BIOCARTA _CELLCYCLE_PATHWAY, KEGG_DNA_REPLICATION, REACTOME_CELL _CYCLE_ CHECKPOINTS, and REACTOMT _G1_S_TRANSITION). d Flow cytometric cell cycle distribution assays to detect the proportion of CRC cells in the G1, S, and G2/M phases after the transfection of SNHG6 siRNAs. e EdU assays were used to determine the cell proliferation ability of SNHG6 siRNAs transfected cells. f Cell cycle-related proteins CyclinD1, CDK4, and CDK6 were detected by western blotting following SNHG6 silencing. g GSEA results were plotted to visualize the correlation between the expression of SNHG6 and genes related to cell apoptosis (HALLMARK_DNA_REPAIR, REACTOME_DNA_REPAIR, and KEGG _APOPTOSIS). h The effect of SNHG6 knockdown on cell apoptosis was analyzed by flow cytometric cell apoptosis assays. i The apoptosis-related proteins caspase-3, cleaved caspase-3, PARP, cleaved PARP and Bax were detected by western blotting after SNHG6 knockdown. Scale bar = 50 μm. *P < 0.05, **P < 0.01 and ***P < 0.001