Fig. 2
Anti-tumor effect of dual PI3K/mTOR inhibition in parental and resistant LMS cell lines. a Growth curves indicating growth inhibition of the three parental (IB112, IB134, and IB136) and resistant (resIB112, resIB134, and resIB136) LMS cell lines after BEZ235 treatment for 72 h. IC50 is indicated in μM. b Percentage of apoptotic cells after BEZ235 treatment for 72 h. Data are presented as the mean ± SEM of three independent experiments. *resistant p < 0.05 vs. parental; **resistant p < 0.01 vs. parental; ***resistant p < 0.001 vs. parental (two-way ANOVA). c Representative signal intensities, obtained by western blotting, for p-S6RPS240/244 were normalized to those for total S6RP in each cell line. Data are presented as the mean ± SEM of three independent experiments