Fig. 6
Effect of EPZ011989 pretreatment on dual PI3K/mTOR sensitivity and on the CSC-like subpopulation in resistant leiomyosarcoma cell lines. a Immunohistochemical staining images of BEZ235-resistant LMS cell line pellets treated with anti-H3K27Me3 antibody (objective magnification, × 10). Percentage corresponds to positively stained cells estimated by a pathologist. b Growth curves indicating growth inhibition of the three resistant (resIB112, resIB134, and resIB136) LMS cell lines after BEZ235 treatment for 72 h. Resistant + EPZ011989 are the cells which were pretreated for 1 week with 10 μM of EPZ011989. IC50 is indicated in μM. p value was calculated by one-way ANOVA. c Percentage of cells with ALDH1 activity, as determined by flow cytometry on resistant cell lines exposed to 10 μM of EPZ011989 for 1 week. Data are presented as the mean ± SEM of three independent experiments. **p < 0.01, two-tailed Student’s t tests