Fig. 7
Effect of BEZ235 and EPZ011989 on resIB136-derived xenografts in Ragγ2C−/− mice and study of expression of H3K27Me3 in patients with sarcoma. a Tumor volume progression curves during 5 weeks of treatment. Mice were randomly pretreated with vehicle or 125 mg/kg of EPZ011989 BID for 2 weeks. Then, in the EPZ011989-pretreated group, mice were randomly treated for 3 weeks with 40 mg/kg of BEZ235 or 125 mg/kg of EPZ011989 BID. The data points represent an average from eight mice (bars, SEM). *p < 0.05, two-way ANOVA. b Kaplan-Meier curves for tumor doubling times. c Immunohistochemical staining images of tumor samples treated with anti-Ki-67, anti-pS6RPser240/244, anti-SOX2, and anti-H3K27Me3 antibodies (objective magnification, × 10). Percentages correspond to positively stained cells estimated by a pathologist. Endothelial cells (positive control) are indicated by black arrows