Fig. 8

Study of expression of H3K27Me3 and heatmap of differentially expressed genes in patients with sarcoma. a Heatmap based on mean, normalized expression of general differentially expressed genes. b Heatmap based on mean, normalized expression of stem cell signaling components. The red and green colors denote high and low intensities, respectively. c Immunohistochemical staining images of three pairs of primary and PI3Kα inhibitor-resistant sarcoma tumor samples treated with anti-H3K27Me3 antibody (objective magnification, × 10). Percentages correspond to positively stained cells estimated by a pathologist. Endothelial cells (positive control) are indicated by black arrows