Fig. 1

CeRNET_CC promotes the stemness of breast cancer cells in vitro. a, b The mRNA levels of CYP4Z1 and the pseudogene CYP4Z2P were detected in breast cancer and normal adjacent tissues via online deposited data (n = 1207, error bars denote the ± SDs, P < 0.01). c KM-plotter (http://kmplot.com) survival curves based on the analysis of a published microarray dataset from breast cancer patients showed the OS probability of patients separated into low and high CYP4Z1 levels. d Venn diagram showing overlap of genes in MCF-7-Z1-UTR and MCF-7-Z2P-UTR cells. e, f Genes with expression levels that were upregulated or downregulated in MCF-7-Z2P-UTR (e) or MCF-7-Z1-UTR (f) cells relative to those in MCF-7 cells. g–i Enrichment of a stem cell signature in a GSEA of genes regulated in MCF-7-Z2P-UTR and MCF-Z1-UTR cells. NES, normalized enrichment score; FDR, false discovery rate. j Functional annotation analysis of genes coordinately activated by CYP4Z1-3′UTR- or CYP4Z2P-3′UTR. k Heat map showing the mean expression values of genes related to epithelial cancer stem cells in MCF-7 cells with CYP4Z2P-3′UTR and CYP4Z1-3′UTR overexpression (fold change > 2 compared with MCF-7 cells, P < 0.05). l Pearson correlation analysis of the expression of CYP4Z1 and the pseudogene CYP4Z2P in breast cancer tissues (n = 1207, P < 0.0001)