Skip to main content
Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: Transcriptional factor six2 promotes the competitive endogenous RNA network between CYP4Z1 and pseudogene CYP4Z2P responsible for maintaining the stemness of breast cancer cells

Fig. 3

CeRNET_CC promotes the stemness of breast cancer cells partly through the hTERT/PI3K/Akt and ERK1/2 pathways. a, b Top-ranked functional pathways enriched by genes in MCF-7-Z1-UTR (a) and MCF-7-Z2P-UTR (b) cells relative to MCF-7 cells. c Phase contrast images of mammospheres formed by MCF-7-Z1-UTR or MCF-7-Z2P-UTR cells with LY-294002, VX-11e, or sihTERT treatment (left); spheres were quantified (right). d Representative FACS profile of cells described in c with CD24 and CD44+ markers. e, f Cells depicted in c were subjected to western blot analysis, and the expression of p-Akt/p-ERK1/2 and stemness markers (ALDH1 and OCT3/4) was then detected. The data are presented as the mean ± SD, n = 3, *P < 0.05 vs. MCF-7-Z1-UTR or MCF-7-Z2P-UTR

Back to article page