Fig. 4
Transcriptional factor six2 induces the progression of ceRNET_CC by directly regulating the transcription of CYP4Z1 and the pseudogene CYP4Z2P. a Diagram of the potential binding sites of six2 on the promoters of CYP4Z1 and the pseudogene CYP4Z2P. b Functional annotation analysis of genes coordinately activated by six2 overexpression in MCF-7 cells. c Enrichment of signaling signatures differentially expressed between MCF-7-six2 and MCF-7 cells based on ChIP-sequencing analysis. d The peak motifs of six2-occupied sites. e Pie chart showing the percentage of six2-occupied genomic regions that are promoter-TSS, exon, intron, or intergenic. f Peak histograms showing six2-occupied CYP4Z1 (left) and pseudogene CYP4Z2P (right) promoters. g, h ChIP-qRT-PCR analysis of six2 occupancy at selected gene promoters in MCF-7 and MCF-7-six2 cells (g) and in MDA-MB-231 and 231-six2 cells (h). Error bars represent the SD from three independent experiments (*P < 0.05, **P < 0.01). i, j Pearson correlation analysis of six2 and the pseudogene CYP4Z2P (i) or CYP4Z1 (j); log2 values of the relative expression levels are presented, n = 32, P < 0.001