Fig. 7

CeRNET_CC is sufficient and necessary for six2-induced effects. a, b Phase contrast images of mammospheres formed by MCF-7-six2 cells with si-CYP4Z1 or si-CYP4Z2P treatment (a) and quantification of spheres (b). The data are presented as the means ± SDs, n = 3, *P < 0.05, **P < 0.01 vs. MCF-7-six2. c Representative FACS profile of cells described in a with CD24⁻ and CD44⁺ markers. d, e Cells depicted in a were subjected to western blot analysis and followed by detecting the expression of p-Akt/p-ERK1/2 (e) and stemness markers (ALDH1 and OCT3/4) (d). f Images of tumors harvested when MCF-7, MCF-7-six2, MCF-7-six2-si-Z1, and MCF-7-six2-si-Z2P cells (left) and MCF-7-Plko-six2, MCF-7-Plko-six2-Z1-UTR, and MCF-7-Plko-six2-Z2P-UTR cells (right) were planted