Fig. 3

SDCSC exosomes are transported to bone marrows and increase neutrophil population in bone marrow. a Representative images of exosomes captured with TEM. Scale = 100 nm. b The size distribution of exosomes by NTA. c Western blots of exosome markers in cells. P, parental cells; S, SDCSCs; WCL, whole cell lysate. d Histograms showing relative exosome secretion in CRC cells. *P < .05. e The representative images for transferring of PKH26-labeled exosomes. Scale bar = 10 μm. f The relative luciferase activity of purified exosomes. g The relative luciferase activity of CT26 cells receiving CT26-SDCSC-released exosomes. GFP-Luc-SDCSC exosome, exosomes from GFP-luciferase-expressing SDCSCs. *P < .05. h The relative luciferase activity in bone marrow cells of normal mice (N = 5), mice with tumors grown from GFP-Luc parental cells (N = 7), and mice with GFP-Luc SDCSC-derived tumors (N = 7). The data represent mean ± SEM. *P < .05. i Western blot of CD81 and FLAG from 10 μg of exosome proteins purified from ectopically FLAG-CD81-expressing SDCSCs and GFP-expressing control cells. j Left: representative images of FLAG-FITC signals in cells. Right: quantification of FLAG-FITC positive cells. ***P < .001