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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer

Fig. 4

The SDCSC exosomes prolong viability of neutrophils and introduce tumor-promoting feature to bone marrow-derived neutrophils. a Representative images of internalized PKH26-labeled SDCSC exosomes in neutrophils. Scale bar = 5 μm. b Relative viability of neutrophils treated with PBS or 20 μg/ml of SDCSC exosomes for 96 h. *P < .05. c Representative images of neutrophils upon treatment at day 3. Scale bar = 25 μm. d Representative flow cytometry results showing early and late apoptotic neutrophils. e A histogram showing the percentage of cells in early apoptosis (Annexin V: positive/ PI: negative cells) and late apoptosis (Annexin V: positive/PI: positive cells). PI, propidium iodide. *P < .05, ***P < .001. f Percentage of neutrophils (left panel) and monocytes (right panel) in bone marrows of mice injected with CT26-SDCSC exosomes or PBS. A total of 45 μg exosomes were injected through tail vein. PBS, mice receiving PBS injection (N = 4); SDCSC exosomes, mice-receiving SDCSC exosome injection (N = 5). The data represent mean ± SEM. **P < .01. g The orthotopic tumorigenicity assay. Left: a table summarizing the tumor incidence. Right: a representative images of grown tumors. The rectangles indicate tumors harvested from peritoneal cavity. Scale bar = 1 cm

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