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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Tumor stem-like cell-derived exosomal RNAs prime neutrophils for facilitating tumorigenesis of colon cancer

Fig. 6

SDCSC-secreted CXCL1 and CXCL2 promote migration of neutrophils for engendering stem-like function in CT26 parental cells by interleukin-1β expression. a Immunoblotting of KC (CXCL1) and MIP-1 (CXCL2) in CRC cells. b Transmigration assay of neutrophils. IgG, normal IgG (10 μg/ml); CXCL1 nAb, neutralizing antibody against CXCL1 (5 μg/ml); CXCL2 nAb, neutralizing antibody against CXCL2 (5 μg/ml). *P < .05, ***P < .001. c RT-qPCR examining IL-1β expression in SDCSC exosome-trained neutrophils. Control CM, complete DMEM medium; CT26 CM, condition medium of parental CT26 cells. The representative results were from two independent assays. *P < .05. d A histogram showing spheroid forming capacity of parental CT26 cells. PBS-CM, condition medium from PBS-treated neutrophils; SDCSC-Ex-CM, condition medium from SDCSC exosome-treated neutrophils; IgG, normal IgG (10 μg/ml); IL-1β nAb, Il-1b neutralizing antibody (10 μg/ml). *P < .05, **P < .01. e The tumorigenicity of CT26 cells cultured under condition medium of neutrophils. Scale bar = 1 cm. f Upper: timeline for showing the sequence of antibody treatment and tumor inoculation. Red arrow, antibody treatment. Lower: a histogram for showing circulating neutrophil concentration at day 0 upon antibody treatment in tumor-free mice. **P < .01. g Representative images of tumors. Scale = 1 cm. h A histogram illustrating tumor weight of harvested tumors. *P < .05. i Expression of MPO in CRCSC signaling on (SNAI1+/IL8+) and off (SNAI1/IL8) CRC patients. ***P < .001. The expression profile of GDC TCGA COAD dataset was used for analysis. j A Kaplan-Meier plotter for showing overall survival of CRC patient with 10% top and bottom MPO expression. k The schematic representation of multistep CRCSC-neutrophil interaction for tumor progression

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