Fig. 7

MYCBP is partially involved in SPAG5 regulated breast cancer cell growth. CCK-8 (a) and colony-forming (b) assays showed that knockdown of MYCBP partially attenuated the enhanced cell proliferation induced by overexpression of SPAG5 in MDA-MB-231 and MDA-MB-468 cells. c Flow cytometry indicates that knockdown of MYCBP partially reverse the increased S to G2 transition induced by overexpression of SPAG5 in MDA-MB-231 and MDA-MB-468 cells. d Flow cytometry indicates that knockdown of MYCBP partially reverse the reduced cell apoptosis induced by overexpression of SPAG5 in MDA-MB-231 and MDA-MB-468 cells. e CCK8 assays showed IC50 values for olaparib in SPAG5 overexpression MDA-MB-231 and MDA-MB-468 cells transfected with MYCBP shRNAs. f MDA-MB-231and MDA-MB-468 cells stably expressing SPAG5 were infected with shRNAs targeting MYCBP. Western blot was used to detect MYCBP, CDC20, Cyclin A2, Cyclin B1, RAD51, BRCA1, and BRCA2 protein expression level. All *p<0.05, **p<0.01, ***p<0.001, n.s. not significant