M28z10 T cells showed strong antitumor activity against GC cell lines in vitro. a Eighteen-hour in vitro killing assays of M28z10 T cells and GFP T cells in multiple GC cell lines, including K562-GL, KATO III-GL, AGS-GL, BGC-823-GL, and MKN-28-GL cells, at each E:T ratio. b Detection of IL-2, IFN-γ, GM-CSF, and granzyme B secretion by M28z10 and GFP T cells after coculture with BGC-823-GL cells for 18 h at an E:T ratio of 1:2. Error bars denote the s.e.m., and the results were compared with an unpaired t test. *P < 0.05, **P < 0.01, and ***P < 0.001. c Detection of multiple cell surface markers on GFP and M28z10 T cells after stimulation with BGC-823 cells at an E:T ratio of 1:2 or no stimulation (gated on CD3 and GFP double positive cells). d Statistical analysis of three independent FACS results. Error bars denote the s.e.m.