Fig. 1
LincK was upregulated during co-culture induced EMT. a Heat map of differential expressed lncRNA from microarray data. 143 upregulated and 154 downregulated lncRNAs were represented in MCF-7 cells co-cultured with hAD-MSCs (for indicated days) compared with MCF-7 cells cultured alone (day 0). b Relative expression of LincK were detected by real-time RT-PCR in MCF-7 cells co-cultured with hAD-MSCs and compared with MCF-7 cells cultured alone (day 0). Relative gene expressions were normalized to GAPDH unless noted otherwise in this study. Results were shown as means ± S.D. of triplicate experiments. c Cytokine secretion of hAD-MSCs before and after co-culture with MCF-7 was detected by ELISA. Results were shown as means ± S.D. d, e Relative expression of LincK in MCF-7 cells after treated with IL6 (50 ng/ml, d) or TGF-β1 (10 ng/ml, e) were detected by qRT-PCR. Data were shown as mean ± S.D. f Schematic annotation of LincK genomic locus on chromosome 8. KB1732A1.1 (uc003ykw.2, top), GASL1 (middle), LincK (bottom) were shown. Rectangles represented exons. g Northern blot of LincK and ACTB transcripts in MCF-7 cells. h Representative images of subcellular localization of LincK detected by RNA-FISH assays in MCF-7 and MCF-7EMT (co-culture induced EMT) cells. DAPI, 4′, 6-diamidino-2-phenylindole. Scale bar, 10 μm. i Fractionation of MCF-7 cells followed by qRT-PCR. GAPDH and ACTB served as cytoplasmic mRNA controls. U1 served as a nuclear RNA control. Bars indicate S.D., n = 3. *p < 0.05; **p < 0.01; ***p < 0.001 (Student’s t test)