Fig. 4

Silenced NOTCH2 abrogates the role of the miR-195-5p inhibitor in CRC cell clone formation, proliferation, migration, and invasion abilities. Representative photomicrographs (a) and quantifications (b) of clone formation assay in HCT116 cells after transfection with the si-NOTCH2, miR-195-5p inhibitor, or si-NOTCH2 together with the miR-195-5p inhibitor for 48 h. Representative photomicrographs (c) and quantifications (d) of EdU immunofluorescence staining assay. Bar = 50 μm. Transwell migration assays of transfected HCT116 cells (e). Bar = 100 μm. Total number of cells in five fields was counted manually (f). Transwell invasion assays of HCT116 cells (g). Bar = 100 μm. Total number of cells in five fields was counted manually (h). Mean ± SD are shown. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001.