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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: RETRACTED ARTICLE: miR-195-5p/NOTCH2-mediated EMT modulates IL-4 secretion in colorectal cancer to affect M2-like TAM polarization

Fig. 5

miR-195-5p inhibits M2-like TAM activation and recruitment. a Fluorescent probe in situ hybridization (FISH) experiment to demonstrate the distribution of miR-195-5p, NOTCH2, and CD163. b Western blots of NOTCH2, Ad-NOTCH2, GATA3, and IL-4 of CRC tissues, miR-195-5p-transfected HCT116 (c), and si-NOTCH2 or IL-4 inhibitor-treated HCT116 (d). e ELISA detected the IL-4 levels in cell supernatants. f–i RT-qPCR tested the macrophage-associated markers after being co-cultured with transfected HCT116. Assays were performed in triplicates. Mean ± SD is shown. Statistical analysis was conducted using one-way ANOVA. *P < 0.05. **P < 0.01. ***P < 0.001

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