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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: Early myeloid-derived suppressor cells (HLA-DR/lowCD33+CD16) expanded by granulocyte colony-stimulating factor prevent acute graft-versus-host disease (GVHD) in humanized mouse and might contribute to lower GVHD in patients post allo-HSCT

Fig. 4

HLA-DR−/lowCD33+CD16 cells could inhibit the development of aGVHD in humanized mouse model. a Protocol for the establishment of aGVHD model through injecting human GPBSC. Sublethally irradiated NSG mice received 5 × 106 human GPBSC with or without 2.5 × 106, 1 × 106, and 5 × 105 G-CSF-mobilized HLA-DR−/lowCD33+CD16 sorted from GPBSC. b Weight Loss, c median survival, and d pathological score in xenografted mice were shown. Mice co-transplanted with HLA-DR−/lowCD33+CD16 (pink, green, and blue, eight mice each group) had improved survival after transplant as compared with GPBSC-injected group (red). For survival, weight change, and disease score: GPBSC + high dose versus GPBSC, GPBSC + medium dose versus GPBSC, and GPBSC + low dose versus GPBSC, p < 0.05. e Representative histology of the target organs harvested on day 22 after transplantation. All results were derived from two pooled independent experiments. Each experiment was performed with G-PBSC from one donor for all recipients. Results were presented and compared with Kaplan-Meier survival curves

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