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Fig. 8 | Journal of Hematology & Oncology

Fig. 8

From: The IAP antagonist birinapant potentiates bortezomib anti-myeloma activity in vitro and in vivo

Fig. 8

Co-administration of TL and Btz suppresses tumor growth in a MM xenograft model. ad NOD/SCID-γ (NSG) mice were subcutaneously (s.c.) inoculated in the right rear flank with 5 × 106 luciferase-expressing U266 cells. TL and Btz were administered via intra-peritoneal (i.p.) injection at a dose of 15 mg/kg (TL) and 0.5 mg/kg (Btz). a Tumors were monitored every other day after i.p. injection with 150 mg/kg luciferin using an IVIS 200 imaging system. Mice were euthanized when tumor length reached 17 mm or humane endpoints were reached; Veh, vehicle. b Tumor size was measured every other day. *P < 0.05 vs Btz; ****P < 0.0001 vs TL. c Kaplan-Meier analysis was carried out to analyze survival. Inset, median survival days. Arrows indicate the time when treatment began (day 18) and was discontinued (day 48). **P < 0.01 vs Btz. d Western blot analysis was performed to monitor the indicated candidate proteins, identified from in vitro studies, in tumors excised from representative mice. Densitometry analysis was performed using ImageJ. Values indicate fold-change of p52 versus untreated control (arbitrarily set as 1.0), after normalization to β-actin. e Mice did not display significant body weight loss (≥ 20%) compared to initial weight (P > 0.05 vs each single agent) or other signs of toxicity over the course of treatment

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