Fig. 4

Trabectedin effects on angiogenesis. a Evaluation of cytokines production and secretion in the supernatant of 3D-spheroids of U266 and monocytes co-cultures treated with 2.5 nM of trabectedin. b Representative image from tubulogenic assay performed with supernatants of treated (2.5 nM of trabectedin) or untreated 3D-spheroids of U266-monocytes co-cultures. The image was analyzed through the “Pipeline 1.4” tool. In blue are reported the segments of neo-vessels/tubes. The histograms below show the tubulogenic potential, quantified by estimating the total tube length and the number of nodal branchpoints (a single pixel connected to 3 or more pixels), of the supernatant derived from 3D Matrigel-spheroids including monocytes co-cultured with 3 different MM cell lines. *p < 0.05