Fig. 5

Histone modifications in the promoter region of interferon target genes differ between 32D-BCR-ABL- and 32D-JAK2V617F-positive cells. a Enrichment analysis of transcription factor binding sites (TFBS) in acetylation (H3K9ac) peaks based on ChIP-seq data. Differentially regulated genes were analyzed for the presence of TFBS in the acetylation peaks, and it was tested if the number of TFBS is significantly different. b 32D-BCR-ABL-transduced (blue) and 32D-JAK2V617F-transduced (red) cells were analyzed for changes of H3K9 acetylation in the promoter region of Stat1, Stat2, Irf1, and Irf9 after 4 h of 1 μM TKI and/or 100 U/ml IFNa treatment by ChIP-PCR and were tested for differences in comparison to the corresponding DMSO-treated cell line. *p < 0.05, **p < 0.01, ***p < 0.001. c Further histone marks such as H3K27 acetylation (active mark), H3K4 tri-methylation (active mark), or H3K27 tri-methylation (repressive mark) were measured by ChIP-PCR (right side) and correlated with Stat1, Stat2, Irf1, and Irf9 mRNA expression (left side) in these 32D-BCR-ABL (blue) and 32D-JAK2V617F (red) cells. Binding was calculated as the percentage of input and shown as mean value ± SD. ChIPs have been performed twice and expression analysis three times in triplicate in independent experiments. Oncogene-expressing 32D cell lines were growing without WEHI for the experimental procedure