Fig. 2
The specific interaction between PBMCs and PD-L1-positive cell lines induced by CD3-HAC. a LDH release assays performed on MDA-MB-231 (PD-L1hi) cells or MCF-7 (PD-L1low) cells infected by various adenovirus in the presence of PBMCs in different effector to target (E:T) ratios for 10 h. b Cytokines including IL-2, IFN-γ, and TNF-α in the co-culture supernatants at E:T ratio (10:1) were detected by ELISA kits. c Flow cytometry analysis on CD69 and CD25 expression on T cells after co-cultured with adenovirus loaded MDA-MB-231 cells for 24 h. d Flow cytometry analysis on the proliferation of PBMCs (labeled with CellTrace™ Far Red) after co-cultured with adenovirus-loaded MDA-MB-231 at E:T radio (10:1) at indicated time. e Representative pictures of PBMC proliferation assay. f Dynamic observation of cell interaction by living cells workstation. Green-colored cells were AdCD3-HAC-transfected MDA-MB-231, and others were PBMCs. Scale bar, 50 μm. SD shown, n ≥ 3. One-way ANOVA with Tukey’s post-test in all cases. ** P < 0.01; ***P < 0.001; ****P < 0.0001; compared with Adtrack group