Fig. 7
Tumor suppression of MSC.CD3-HAC.E1A in combination with 5-FU against MDA-MB-231 lung metastasis. a Design and timeline for tumor therapy. BALB/c nude mice were injected with MDA-MB-231-Luc cells (Luc-231, 1 × 106 per mouse) via tail vein. Seven days later (day 1), MSCs (1 × 106 per mouse) were administrated intravenously into the Luc-231 tumor-bearing mice. Then, mice received intravenous injection of PBMCs (5 × 106 per mouse) on day 4. Besides, 5-FU was given (i.p., 20 mg/kg) every other day along the treatment. The same treatment was performed another two times as indicated. i.p., intraperitoneal. b IVIS imaging of MDA-MB-231-Luc cells were shown for all groups. IVIS imaging was taken before (day 0) and after MSCs treatment (day 10 and day 20). Quantification of luciferase signals in the lung on day 10 (c) and day 20 (d). e Mouse survival after MSC.CD3-HAC.E1A treatment (n = 7 per group). In c, relative growth index = luciferase read on day 10 /luciferase read on day 0. In d, lung metastasis index = log10 [(luciferase read of the tested mice)/(luciferase read of average for tumor-free mice)]. *P <0.05 and **P <0.01 compared with the PBS group. In e, P = 0.0108, MSC.CD3-HAC.E1A+PBMC versus PBS; P = 0.0161, MSC.CD3-HAC.E1A+PBMC+5-FU versus PBS. Median survival (days): PBS, 60; 5-FU, 61; MSC+PBMC, 56; MSC.CD3-HAC.E1A+PBMC, 69; MSC.CD3-HAC.E1A+PBMC+ 5-FU, 89