Fig. 3

CD73 triggers EMT in HCC. a Representative images of HCC cells with CD73 downregulation or overexpression. Cells were stained by FITC-phalloidin; scale bar 20 μm. b Expressions of EMT-related markers in HCC cells with CD73 downregulation or overexpression by RT-PCR assays. c Expressions of EMT-related markers in HCC cells with CD73 downregulation or overexpression by WB assays. d Expressions of CD73, E-Cadherin, N-Cadherin, and Vimentin in HCC cell lines with CD73 downregulation or overexpression by immunofluorescence staining; scale bar 200 μm. e Representative IHC images of CD73, E-Cadherin, and N-Cadherin expressions in tumor tissues derived from HCC cell lines as indicated. f Representative IHC images of CD73, E-Cadherin, and N-Cadherin expressions in consecutive tissue sections of clinical HCC samples. g Correlations between CD73 and E-Cadherin or N-Cadherin in clinical HCC samples were analyzed by Spearman’s rank correlation test. PCR assays were performed in triplicate. “N.S.” indicated not significant; asterisk indicated P < 0.050, t tests were used