Fig. 6

CD73 is regulated by miR-193b. a Putative binding site of miR-193a/miR-193b and CD73. b Expression of CD73 in Hep3B and HCCLM3 cells after miR-193b mimic transfection as detected by RT-PCR and WB assays; asterisk indicated P < 0.050 when compared with parental group, and t tests were used. c Effect of miR-193b on the activity of CD73 mRNA 3′UTR was evaluated by luciferase reporter assays. d Comparison of miR-193b expression level between paratumor and tumor tissues determined by RT-PCR assays; asterisk indicated P < 0.050, n = 25, and t tests were used. e Correlation of miR-193b and CD73 mRNA in clinical HCC samples was analyzed by Spearman’s rank correlation test, n = 25. f Evaluations of proliferation capacities of indicated HCCLM3 (left) and Hep3B cells (right) by CCK-8 assays; “N.S.” indicated not significant; asterisk indicated P < 0.050, and t tests were used. g Evaluations of migration capacities of indicated HCCLM3 (left) and Hep3B cells (right) by wound healing assays; asterisk indicated P < 0.050, and t tests were used. h Evaluations of invasion capacities of indicated HCCLM3 (left) and Hep3B cells (right) by wound healing assays; asterisk indicated P < 0.050, t tests were used. i Evaluations of EMT-related genes and pAKT expression levels of indicated HCCLM3 (left) and Hep3B cells (right) by WB assays. In vitro experiments were performed in triplicate