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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Downregulation of CD73 associates with T cell exhaustion in AML patients

Fig. 5

CD73− CD8+ T cells from AML patients showed functional defects in cytokine production and high susceptibility to apoptosis. a Intracellular cytokine production of IL-2, TNF-α, and IFN-γ by CD73− and CD73+ CD8 T cells from AML patients (n = 13) upon in vitro anti-CD3/anti-CD28 stimulation. P values were obtained by paired t test (IL-2, IFN-γ) or Wilcoxon matched-pairs signed rank test (TNF-α). b CD8 T cells were purified from PBMC of HLA-A*0201 AML patients at initial diagnosis. Then, they were co-cultured with T2 cells (used as antigen-presenting cells) that were pulsed with HLA-A*0201-binding WT-1126-134 peptide for 6 days. Then, cells were collected and intracellular staining was performed. Shown are the expression of IL-2, TNF-α, and IFN-γ in CD73− vs. CD73+ leukemia-reactive CD8 T cells assessed by flow cytometry. In the left are representative flow cytometry data. In the right are the statistical summary plots; each dot indicates one patient. P values were obtained by paired Student’s t test or Wilcoxon signed-rank test. c, d Expression of CD95 (c) and Annexin V (d) on CD73− and CD73+ CD8 T cells from AML patients (n = 13). P values were obtained by paired t test

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