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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: 2B4 costimulatory domain enhancing cytotoxic ability of anti-CD5 chimeric antigen receptor engineered natural killer cells against T cell malignancies

Fig. 1

Construction of CD5 CAR and preparation of CAR-NK cells. a Schematic diagram of lentiviral CAR expression plasmids. b Representative flow cytometry analysis showing the expression of CARs on NK-92 cells. c SFI of F (ab’)2 on VEC-NK, BB.z-NK, and 2B4.z-NK cells (n = 3; n.s., no significance). d CCK8 assay of cell proliferation in VEC-NK, BB.z-NK, and 2B4.z-NK cells (n = 3; ***p < 0.001). e Representative flow cytometry analysis showing basal apoptosis of VEC-NK, BB.z-NK, and 2B4.z-NK cells. f Quantification and statistical analysis of the data in e (n = 3; *p < 0.05; ***p < 0.001; n.s., no significance). VEC: VEC-NK; BB.z: BB.z-NK; 2B4.z: 2B4.z-NK

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