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Fig. 1 | Journal of Hematology & Oncology

Fig. 1

From: The long noncoding RNA H19 promotes tamoxifen resistance in breast cancer via autophagy

Fig. 1

Increased autophagy promotes tamoxifen resistance. a Representative images of the colony formation assays using MCF7 or MCF7/TAMR cells under 0, 5, and 10 μM tamoxifen treatment for 14 days. The bar graphs show the quantification of the colony formation assay data. The data are presented as the mean ± SD of three independent experiments. Student’s t test was used for statistical analysis. b Autophagic vacuoles stimulated by 10 μM tamoxifen for 12 h in MCF7 or MCF7/TAMR cells were analyzed using a Cyto-ID autophagy detection assay. Stv: starvation. The data are presented as the mean ± SD of three independent experiments. Student’s t test was used for statistical analysis. c. Both MCF7 cells and MCF7/TAMR cells were exposed to 10 μM tamoxifen for 12 h. Western blotting was performed to examine the expression of Beclin1 and LC3. The value next to each blot is the quantification of the relative expression of the indicated band normalized to GAPDH expression. d. Representative images of the colony formation and cell apoptosis assays using MCF7/TAMR cells that were cultured in blank, tamoxifen, 3-MA, CQ, or tamoxifen combined with 3-MA or CQ media. e. Bar graphs showing the quantification of the colony formation assay data. The data are presented as the mean ± SD of three independent experiments. Student’s t test was used for statistical analysis. f. Bar graphs showing the percentage of apoptotic cells. The data are presented as the mean ± SD of three independent experiments. Student’s t test was used for statistical analysis. **P < 0.01, ***P < 0.001, and ****P < 0.0001 compared with the control group. n.s. indicates no significant difference

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