Fig. 6

Knockdown of H19 lncRNA suppresses autophagy and restores tamoxifen sensitivity in vivo. a–c The effect of H19 expression on in vivo tumorigenicity was evaluated using a xenograft nude mouse model. Tumors formed in nude mice (a), isolated subcutaneous tumors (b), and tumor growth curves (c); n = 5 mice/group. The error bars indicate the SD. Student’s t test was used for statistical analysis. d mRNA expression of H19 in the xenografts was analyzed by qRT-PCR. The error bars indicate the SD. Student’s t test was used for statistical analysis. e Representative images of immunohistochemical (IHC) staining for Beclin1, LC3, and P62 in the xenografts. The box graphs show the quantification of IHC staining. Scale bars 50 μm. Chi-square test was used for statistical analysis. f Western blotting was conducted to analyze the protein expression levels of Beclin1 and LC3 in xenograft tumors. The value next to each blot is the quantification of the relative expression of the indicated band normalized to GAPDH expression. *P < 0.05, **P < 0.01 compared with the control group