Fig. 4
From: Functional diversity of inhibitors tackling the differentiation blockage of MLL-rearranged leukemia
Phagocytosis of E.coli particles after inhibitor-induced differentiation. a qRT-PCR analysis of representative surface markers and genes associated with hematopoietic differentiation in MOLM-13 cells after 7 days of treatment with BAY-155 (3 μM), OTX015 (0.2 μM), EPZ-5676 (6 μM), BAY 1251152 (0.05 μM), and Brequinar (3 μM). Data presented is an average of three biological replicates normalized to vehicle control (DMSO). n.e. not expressed. b Flow cytometry scatter plots (1st and 3rd left column) showing the distribution of CD11b (APC) and E.coli (FITC labeled) staining of viable MOLM-13 cells after 7 days of treatment with BAY-155, (3 μM) OTX015 (0.2 μM), EPZ-5676 (6 μM), BAY 1251152 (0.05 μM), and Brequinar (3 μM). Representative experiment of three biological replicates is shown. Immunofluorescence staining (2nd and 4th left column) of MOLM-13 cells after 7 days of treatment with BAY-155, (3 μM) OTX015 (0.2 μM), EPZ-5676 (6 μM), BAY 1251152 (0.05 μM), and Brequinar (3 μM). Representative merge image of CD11b (red), E.coli particles (yellow), and nucleus (blue) is shown