Fig. 3
From: Glyphosate induces benign monoclonal gammopathy and promotes multiple myeloma progression in mice
Glyphosate-treated Vk*MYC mice developed progressive plasma cell neoplasms. a Representative flow cytometry plots detecting cell surface markers CD138 (Y-axis) and B220 (X-axis) in splenocytes (upper panel) and bone marrow cells (lower panel). The numbers on the axes denoted the log10 values of fluorescence. The numbers in the inserts show the percentage of CD138highB220- cells in the entire cell population. b, c Bar graphs of the percentages of CD138+B220- and B220+ cells from the spleen (b) and bone marrow (c). Data were analyzed by one-way ANOVA. d Confocal microscopy images identifying Ki67+ (green) and CD138+ (red) expression with nuclear DAPI staining of cells in the spleen of a representative WT (upper panel) and Vk*MYC mouse (lower panel), both treated with glyphosate. Scale bar = 10 μm. e Confocal microscopy images identifying Ki67+ (green) and CD138+ (red) expression with nuclear DAPI staining of cells in the bone marrow of WT (upper panel) and Vk*MYC mice (lower panel) treated with glyphosate. Scale bar = 10 μm. n = 10 mice per group. *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001