Skip to main content
Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: Dual inhibition of IGF-IR and ALK as an effective strategy to eradicate NPM-ALK+ T-cell lymphoma

Fig. 5

Effects of combining treatment with PPP and ASP3026 on survival proteins in NPM-ALK+ T cell lymphoma cells. Western blotting shows that at 48 h, treatment with a low concentration of PPP (0.375 μM) alone was associated with downregulation of IGF-IR phosphorylation in Karpas 299 and DEL cells. In addition, ASP3026 (1.0 μM) alone induced a notable decrease in the phosphorylation of NPM-ALK, IGF-IR, and STAT3. Nevertheless, the addition of the low concentration of PPP to ASP3026 induced dramatic downregulation of phosphorylated IGF-IR, NPM-ALK, and STAT3. Occurrence of apoptosis was biochemically supported by an increase in cleaved PARP (C-PARP) and a simultaneous decrease in non-cleaved PARP levels. These observations were remarkably more pronounced after the combined treatment. β-Actin indicates equal protein loading

Back to article page