Fig. 5

Methylation of GAPDH is important for PRMT3-induced metabolic reprogramming. a ECAR was measured with Seahorse XF24 Flux analyzer in GFP- and GFP-PRMT3-overexpressing PANC-1 cells with or without SGC707 treatment. Basal ECAR measurement was measured in XF assay medium without glucose, following by the addition of glucose (10 mM), oligomycin (1 μM), and 2-DG (50 mM). Error bars, SEM. n = 3. Column statistics of ECAR is shown in the right panel. *p < 0.05, **p < 0.01, ***p < 0.001. N.S., not significant. b OCR was measured in GFP- and GFP-PRMT3-overexpressing PANC-1 cells with or without SGC707 treatment. Basal OCR was measured in the Seahorse XF24 Flux analyzer. Measurements were performed by injecting oligomycin (1 μM), FCCP (0.5 μM), and rotenone (2 μM). Error bars, SEM. n = 3. Column statistics of OCR is shown in the right panel. *p < 0.05, **p < 0.01, ***p < 0.001. N.S., not significant. c ECAR was measured in PANC-1 cells with co-expression of GFP-, GFP-PRMT3, Flag-GAPDH-WT, or Flag-GAPDH-R248K mutant. Error bars, SEM. n = 3. Column statistics of ECAR is shown in the right panel. *p < 0.05, **p < 0.01, ***p < 0.001. N.S., not significant. d OCR was measured in PANC-1 cells with co-expression of GFP-, GFP-PRMT3, Flag-GAPDH-WT, or Flag-GAPDH-R248K mutant. Error bars, SEM. n = 3. Column statistics of OCR is shown in the right panel. *p < 0.05, ***p < 0.001