Fig. 3

LINC01123 enhances NSCLC cells metabolic rewiring in vitro. a, b ¹⁸F-FDG uptake, lactate release, and ATP production level were determined in H1299 and A549 cells. c, d Relative expression levels of metabolic genes in H1299 cells after being transfected with si-1123 and in A549 cells after transfection with pcDNA-1123. e–h HK2 and LDHA protein expression, LDH enzyme activity, and ROS accumulation were examined in H1299 and in A549 cells. i–l The quantification of OCR and ECAR measured by Seahorse XF assays in H1299 and A549 cells with LINC01123 knockdown (i, k) or overexpression (j, l).The OCR curves were treated with oligomycin, FCCP, and rotenone/antimycin A, while the ECAR curves were treated with glucose, oligomycin, and 2-DG. Data shown are mean ± SD (n = 3). (*P < 0.05, **P < 0.01, ***P < 0.001)