Fig. 3
From: RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia
Cooperation of ASXL1-R693X and RUNX1-R135T in mouse BM cells increased myeloid colony. a, b Colony-forming potentials of mouse BM cells transduced with indicated plasmids in methylcellulose colony-forming media; representative images of colonies in the second round plating were shown. Colonies were photographed and counted manually, original magnification × 100. Columns represent the number of serially replated colonies. c The proportion of CFU colonies of second-round serial replating. d Cytospin smeared preparations of cultured cells in the first plating colony assays by Wright–Giemsa staining was shown. Error bars represent the mean ± SD from duplicated cultures. *P < 0.05, **P < 0.03, ***P < 0.01