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Fig. 4 | Journal of Hematology & Oncology

Fig. 4

From: RUNX1 mutations promote leukemogenesis of myeloid malignancies in ASXL1-mutated leukemia

Fig. 4

ASXL1-R693X collaborates with RUNX1-R135T in myeloid transformation. a Kaplan–Meier curve shows the survival of mice transplanted with BM cells transduced with empty vector, ASXL1-R693X, RUNX1-R135T, and combined expression of ASXL1-R693X and RUNX1-R135T. P values were calculated using a log-rank test. b Significant hepatomegaly and splenomegaly in diseased mouse transplanted with BM cells transduced with ASXL1-R693X and RUNX1-R135T. A representative photograph is shown. c, d Quantification of hepatomegaly (c) and splenomegaly (d) in mice transduced with EV, ASXL1-R693X, RUNX1-R135T, and coexisted mutations of ASXL1 and RUNX1 are shown (n = 5). e Peripheral blood counts of transduced mice (n = 5 for each group). f Representative peripheral blood smears from mice transduced with both ASXL1-R693X and RUNX1-R135T showing dysplastic neutrophils (i–ii) and hypersegmented nuclei (iii) (upper panel, magnification × 400); BM and spleen cytospin smears showing abnormal myeloid cells (lower panel, magnification × 400). g Flow cytometric analyses of myeloid progenitors in BM cells derived from mice transplanted with ASXL1-R693X, RUNX1-R135T, combined expression of ASXL1-R693X and RUNX1-R135T, and EV control (n = 4 of each group). P value showing calculated as indicated in the figures

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