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Fig. 5 | Journal of Hematology & Oncology

Fig. 5

From: MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells

Fig. 5

The investigation of MAGE-A1 activity in the development of LUAD in vitro and in vivo. a MAGE-A1 knockdown (shMAGE) and MAGE-A1 overexpression (OEMAGE) models were successfully constructed using the PC9 cell line. For shMAGE, qPCR and western blotting analyses showed that MAGE-A1 expression levels in shMAGE1 (sh1), shMAGE2 (sh2), and shMAGE3 (sh3) were significantly reduced. *Significant difference in MAGE-A1 expression in shMAGE cell line compared with the wild-type (WT) cell line. p < 0.05. For OEMAGE, qPCR and western blotting analyses showed that the MAGE-A1 expression level in OEMAGE (OE) was significantly elevated. *Significant difference in MAGE-A1 expression in the OEMAGE cell line compared with the WT cell line. p < 0.05. bd CCK-8, wound healing, and transwell assays demonstrated that shMAGE drastically inhibited PC9 cell proliferation, migration, and invasion, while OEMAGE significantly augmented PC9 cell proliferation, migration, and invasion in vitro. *Significant difference in cell proliferation, migration, and invasion in shMAGE or OEMAGE cell lines compared with WT cell lines. e, f Xenograft tumors developed from OEMAGE cells grew significantly faster than those developed from shMAGE cells. g The volume of shMAGE tumors was much smaller than that of OEMAGE tumors at 48 days after cell inoculation. *Significant difference in tumor volume in tumors from shMAGE or OEMAGE cell lines compared with tumors from WT cell lines

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