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Fig. 8 | Journal of Hematology & Oncology

Fig. 8

From: MAGE-A1 in lung adenocarcinoma as a promising target of chimeric antigen receptor T cells

Fig. 8

The anti-tumor activity of mCART against LUAD was investigated by mouse xenografts in vivo. a Flow diagram of the in vivo test. For mCART preparation, PBMCs from a healthy donor were collected on day 10, and lentivirus infection was performed on day 6. Mice were subcutaneously implanted with luciferase-expressing H1299 cells until the tumor volume reached approximately 100 mm3 and then randomly divided into three groups (mCART, unrelated-CART, and T). On days 0, 3, and 6, mice received intravenous treatment with mCART (1 × 107), unrelated-CART, and T therapy. On days 2, 5, 8, 13, and 20, bioluminescent images were recorded. On day 27, all mice were killed, and the tumors from each animal were removed, measured, and weighed individually. b, c Serial bioluminescence imaging and tumor signal in mice was recorded to follow tumor progression. *Significant difference in bioluminescence imaging in the mCART group compared with that in the T group. d Serial volume of xenograft tumors. *Significant difference in the volume of xenograft tumors in the mCART group compared with the T group. e, f Comparison of xenograft tumor weight and morphology on day 27. *Significant difference in weight of xenograft tumors in the mCART group compared with the T group. g Comparison of CD3 expression in xenograft tumors by IHC analysis

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