Fig. 3

S100A11 membrane translocation participates in stiffness-induced mesenchymal shift in HCC cells. a Overexpression of S100A11 or eIF4E promoted an increase in N-cadherin, vimentin, and α-SMA expression and a decrease in E-cadherin expression in HCC cells grown on high stiffness substrate. b Knockdown of S100A11 or eIF4E partially attenuated high stiffness-induced EMT in HCC cells. c S100A11 distribution in HCC cells transfected with pEGFP-OE-S100A11 grown on different stiffness substrates. L, low-stiffness substrate, 6 KPa; M, medium-stiffness substrate, 10 KPa; H, high-stiffness substrate, 16 KPa. d S100A11 expression at membrane protein and total protein level in HCC cells under different stiffness stimulations. e Knockdown of integrin α5 or integrin β1 partially inhibited S100A11 membrane translocation, but little effect on S100A11 expression at total protein level. f Higher matrix stiffness strengthened S100A11 interaction with p67 phox and p47 phox. g ROS production in HCC cells transfected with pEGFP-OE-S100A11 or pFU-GW-shRNA-S100A11 in presence or absence of DPI under different stiffness stimulations. h Snail expression in HCC cells transfected with pEGFP-OE-S100A11 or pFU-GW-shRNA-S100A11 in presence or absence of DPI. Error bars indicate SD. *P < 0.05, **P < 0.01, ***P < 0.001