Fig. 6
From: APRIL and BAFF: novel biomarkers for central nervous system lymphoma
APRIL and BAFF increase cell survival during MTX treatment and induce chemotaxis in lymphoma cells in vitro. a In full growth medium (FGM), cell proliferation is increased compared to serum-free medium (SFM). APRIL and BAFF did not affect the proliferation of HKBML or OCI-Ly10 cells when added to SFM. b Under MTX exposition in SFM, the addition of APRIL significantly increased the tumor cell viability of HKBML cells (P < .01), while the addition of BAFF significantly increased the tumor cell viability of OCI-Ly10 cells (P < .05). The APRIL- and BAFF-neutralizing TACI-Fc abrogate these APRIL- and BAFF-mediated effects. c OCI-Ly10 and HKBML cells exhibit increased chemotaxis to BAFF, and HKBML cells also to APRIL in a dose-dependent manner. Chemotaxis index is defined as migrated cells after addition of chemokine normalized to the number of spontaneously migrated cells in SFM. *P < .05; **P < .01; ***P < .001