Fig. 2

CircAF4 plays an oncogenic role in MLL-AF4 leukemia in vitro. a Schematic illustration showing the junction site of circAF4 and two targeted siRNAs. qRT-PCR for circAF4 and AF4 mRNA in RS4;11 (b) and SupB15 (c) cells treated with two siRNAs as described above. NS, no significant. ***P < 0.001. Proliferation of RS4;11 (d) and SupB15 (e) cells transfected with the above two siRNAs assessed using a CCK-8 kit (Dojindo, Japan) at the indicated days. NS, no significant. ***P < 0.001. RS4;11 (f) and SupB15 (g) cells were exposed to 10 μM and 3 μM dexamethasone, respectively, for 18 h before collection at 48 h after transfection. A representative experiment is shown. NS, no significant. ***P < 0.001