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Fig. 3 | Journal of Hematology & Oncology

Fig. 3

From: circRNA circAF4 functions as an oncogene to regulate MLL-AF4 fusion protein expression and inhibit MLL leukemia progression

Fig. 3

CircAF4 promotes leukemogenesis in vivo. a qRT-PCR confirmation of knockdown of circAF4 by lentiviral constructs in RS4;11 cells. Data are shown by the mean ± SEM of three experiments. ***P < 0.001. b Wright-Giemsa-stained cytospins for bone marrow samples isolated from mice engrafted with human RS4-11 cells co-transfected with the sh-NC and sh-circAF4. GFP+ cells in mice are indicated by black arrows. c Representative photographs of the spleen from control and RS4-11 treated mice. The spleen size and weight were notably reduced in sh-circAF4-treated mice (n = 3). *P < 0.05. d Hematoxylin and eosin staining showed the infiltration of leukemic cells in the indicated organs of mice engrafted with RS4-11 expressing the sh-circAF4 compared to control mice. GFP+ cells in tissue are indicated by black arrows. Staining was observed under a × 40 objective lens. Scar bars, 50 μm. e The flow cytometry result showed the substantially decreased level of GFP+ cells in blood and bone marrow samples form the mice treated with circAF4-knockdown RS4-11 cells versus control. These effects were accompanied by reduced blast infiltration of the spleen in circAF4-knockdown mice. **P < 0.01 and ***P < 0.001. f Kaplan-Meier survival curves of NOD-SCID mice transplanted with sh-NC and sh-circAF4 treated RS4-11 cells (n = 5 mice per group). P values were calculated using Log-rank (Mantel-Cox) test

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