Fig. 1
Longitudinal analysis of copy number aberrations in stable and progressive CLL patients. Genome-Wide Human SNP Arrays 6.0 (Affymetrix) was used to genotype patient DNAs at the two sequential sampling points, first time point (FTP), and last time point (LTP). Data were processed using the Rawcopy package, and paired segments (PSs) were identified between FTP and LTP of each patient (see Methods in Additional file 2). Aberrant loci were identified by varying the percentage of cancer cells (f) using two sets of threshold on log ratio (LogR), one for DNA amplification (LogRA) and one for DNA deletion (LogRD). a The percentage of PSs and b their slopes (∆LogRLTP, FTP/∆tLTP, FTP) are shown as mean (solid line) and standard deviation (shades) based on no change, acquisition, or loss of aberration as a function of f. No change of aberration: the considered locus was aberrant both in FTP and in LTP; acquisition of aberrations: the considered locus was aberrant only in LTP; loss of aberrations: the considered locus was aberrant only in FTP. Red colors indicate the P-CLLs; blue the S-CLLs. p values’ graphs (lower panel) report the Mann-Whitney U test for each f, significance was defined as p < 0.050