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Fig. 6 | Journal of Hematology & Oncology

Fig. 6

From: Preclinical efficacy for a novel tyrosine kinase inhibitor, ArQule 531 against acute myeloid leukemia

Fig. 6

Immunoblot analysis for MCL1 modulation in MOLM-13 FLT3-ITD cell line (representative of two independent blots). Cells were serum starved and treated for 20 h with ARQ 531 alone or in combination with venetoclax. Twenty micrograms of total protein lysate was loaded per lane. GAPDH used as loading control. Band densitometry is done using ImageJ and quantified relative to GAPDH and DMSO treatment (a). In vitro analysis for additive or synergistic activity for ARQ 531 and venetoclax combination. Representative of three MTS proliferation assays. The MOLM-13 cell line was treated with increasing doses of ARQ 531 or venetoclax single agents or a combination of both for 72 h. Three biological replicates were analyzed using Combenefit software implementing the mathematical models for Highest Single Agent (HSA), Loewe, and Bliss (b). Table represent the concentrations with evidence of synergy (calculated ratio of proliferation with single agent alone vs. in combination > 1 via mixed effects model—see Additional file 11: Supplemental Information 1 for more details, p value ≤ 0.05) (c)

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