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Fig. 2 | Journal of Hematology & Oncology

Fig. 2

From: Aspartate β-hydroxylase promotes pancreatic ductal adenocarcinoma metastasis through activation of SRC signaling pathway

Fig. 2

ASPH activates SRC signaling in pancreatic cancer. a A direct physical interaction of ASPH with ADAM12 or ADAM15 was detected by co-IP in HEK293 cells. b Endogenous ASPH-induced SRC activation (Y416) was efficiently inhibited by dasatinib. ADAM12 or ADAM15 overexpression substantially activated SRC was blocked by dasatinib. c ASPH KO or ADAM12/ADAM15 knock-down markedly downregulated SRC signal. d, e Exogeneous or f, g endogenous ASPH-mediated activation of SRC signaling was inhibited by both SMI and dasatinib. h 3-D tumor spheroid invasion in response to dasatinib. i ECM degradation/remodeling in response to dasatinib. j 3-D pancreatosphere formation in response to dasatinib. k Transendothelial migration and intravasation/extravasation. l Invasion through basement membrane and subsequent pancreatosphere formation cells in response to dasatinib. *p < 0.05; **p < 0.01; ***p < 0.001

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